Roche LightCycler 480 with 384-Well Plate: Advanced Real-Time PCR System

 The Roche LightCycler 480 is a high-performance real-time PCR system designed for precise, high-throughput nucleic acid analysis. With its 384-well plate format, this instrument is ideal for laboratories requiring rapid and reliable quantitative PCR (qPCR) for gene expression studies, genotyping, and mutation detection.

Key Features

1. High Throughput with 384-Well Format

The Roche LightCycler 480 supports 384-well plates, allowing researchers to process a large number of samples in a single run. This is particularly useful for high-throughput applications such as screening assays and large-scale gene expression studies.

2. Precise Temperature Control

Equipped with an advanced thermal block, the system provides uniform and accurate temperature control, ensuring reproducibility across wells. The Thermal Gradient feature enables optimization of PCR conditions within a single run.

3. Multiplexing Capability

The instrument supports multiplex qPCR, allowing simultaneous detection of multiple targets in a single reaction. This reduces reagent consumption and increases efficiency in molecular biology workflows.

4. Sensitive and Accurate Detection

The LightCycler 480 utilizes state-of-the-art optical detection technology, ensuring high sensitivity and accuracy. The system supports a variety of fluorescent dyes and probes, making it versatile for different qPCR applications.

5. User-Friendly Software

Roche provides intuitive software for data acquisition, analysis, and interpretation. Features such as automatic baseline correction and advanced melting curve analysis enhance usability and data quality.

6. Wide Application Range

The system is widely used for:

• Gene expression quantification

• SNP genotyping

• Mutation analysis

• Pathogen detection

• Copy number variation studies

Benefits of Using the LightCycler 480 with 384-Well Plate

• Increased Efficiency: Process more samples in less time, reducing workflow bottlenecks.

• Cost-Effective: Multiplexing capabilities save reagents and reduce per-sample costs.

• Reliable Results: Advanced temperature control and optical detection ensure high-quality data.

• Scalability: Ideal for both small-scale research and large-scale clinical applications.

Conclusion

The Roche LightCycler 480 with a 384-well plate is a powerful real-time PCR system designed to meet the demands of high-throughput laboratories. With its precision, sensitivity, and efficiency, it is a preferred choice for researchers and clinicians working in molecular diagnostics, biotechnology, and genetic research.